Efficient transient gene expression system using buckwheat hypocotyl protoplasts for large-scale experiments.

ABSTRACT: Buckwheat (Fagopyrum esculentum) is cultivated worldwide and its flour is used in a variety of food products. Although functional analyses of genes in buckwheat are highly desired, reliable methods to do it have yet to be developed. In this study we established a simple and efficient transient gene expression system using buckwheat protoplasts isolated from young hypocotyls using 96-well plates as a high-throughput platform. The transformation efficiency was comparable with that of similar systems, such as Arabidopsis mesophyll protoplasts. Stable results were obtained in a typical example of the experiment to examine transcription factor activity. This system shows potential for the large-scale analysis of gene function using protoplast isolated from fewer and younger plants than the conv

SUBMITTER: Sakamoto S 

PROVIDER: S-EPMC7180138 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature