One-Step Enrichment of Intact Glycopeptides From Glycoengineered Chinese Hamster Ovary Cells.

ABSTRACT: Recently, the glycoproteomic analysis of intact glycopeptides has emerged as an effective approach to decipher the glycan modifications of glycoproteins at the site-specific level. A rapid method to enrich intact glycopeptides is essential for the analysis of glycoproteins, especially for biopharmaceutical proteins. In this study, we established a one-step method for the rapid capture of intact glycopeptides for analysis by mass spectrometry. Compared to the conventional sequential enrichment method, the one-step intact glycopeptide enrichment method reduced the sample preparation time and improved the detection of intact glycopeptides with long sequences or non-polar amino acids. Moreover, an increased number of glycosite-containing peptides was identified by the one-step method compared

SUBMITTER: Yang G 

PROVIDER: S-EPMC7180227 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature