Influence of the Sample Preparation Method in Discriminating Candida spp. Using ATR-FTIR Spectroscopy.

ABSTRACT: Several studies have investigated the capacity of ATR-FTIR spectroscopy for fungal species discrimination. However, preparation methods vary among studies. This study aims to ascertain the effect of sample preparation on the discriminatory capacity of ATR-FTIR spectroscopy. Candida species were streaked to obtain colonies and spectra were collected from each preparation type, which included: (a) untreated colonies being directly transferred to the ATR crystal, (b) following washing and (c) following 24-h fixation in formalin. Spectra were pre-processed and principal component analysis (PCA) and K-means cluster analysis (KMC) were performed. Results showed that there was a clear discrimination between preparation types. Groups of spectra from untreated and washed isolates clustered separate

SUBMITTER: Pebotuwa S 

PROVIDER: S-EPMC7180699 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature