Cardiomyocyte Contractility and Autophagy in a Premature Senescence Model of Cardiac Aging.

ABSTRACT: Globally, cardiovascular diseases are the leading cause of death in the aging population. While the clinical pathology of the aging heart is thoroughly characterized, underlying molecular mechanisms are still insufficiently clarified. The aim of the present study was to establish an in vitro model system of cardiomyocyte premature senescence, culturing heart muscle cells derived from neonatal C57Bl/6J mice for 21 days. Premature senescence of neonatal cardiac myocytes was induced by prolonged culture time in an oxygen-rich postnatal environment. Age-related changes in cellular function were determined by senescence-associated β-galactosidase activity, increasing presence of cell cycle regulators, such as p16, p53, and p21, accumulation of protein aggregates, and restricted pr

SUBMITTER: Haseli S 

PROVIDER: S-EPMC7180990 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature