Outlier Profiles of Atomic Structures Derived from X-ray Crystallography and from Cryo-Electron Microscopy.

ABSTRACT: Background: As more protein atomic structures are determined from cryo-electron microscopy (cryo-EM) density maps, validation of such structures is an important task. Methods: We applied a histogram-based outlier score (HBOS) to six sets of cryo-EM atomic structures and five sets of X-ray atomic structures, including one derived from X-ray data with better than 1.5 Å resolution. Cryo-EM data sets contain structures released by December 2016 and those released between 2017 and 2019, derived from resolution ranges 0-4 Å and 4-6 Å respectively. Results: The distribution of HBOS values in five sets of X-ray structures show that HBOS is sensitive distinguishing sets of X-ray structures derived from different resolution ranges-higher than 1.5 Å, 1.5-2.0 Å, 2.0-2.5 Å, 2.5-3.0

SUBMITTER: Chen L 

PROVIDER: S-EPMC7181022 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature