Site-Selective and Rewritable Labeling of DNA through Enzymatic, Reversible, and Click Chemistries.

ABSTRACT: Current methods for bioconjugation rely on the introduction of stable linkers that lack the required versatility to perform sequential functionalizations. However, sequential manipulations are an increasing requirement in chemical biology because they can underpin multiple analyses of the same sample to provide a wider understanding of cell behavior. Here, we present a new method to site-selectively write, remove, and rewrite chemical functionality to a biomolecule, DNA in this case. Our method combines the precision and robustness of methyltransferase-directed labeling with the reversibility of acyl hydrazones and the efficiency of click chemistry. Underpinning the method is a new S-adenosyl-l-methionine derivative to site-selectively label DNA with a bifunctio

SUBMITTER: Wilkinson AA 

PROVIDER: S-EPMC7181315 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature