Unbiased Identification of the Liposome Protein Corona using Photoaffinity-based Chemoproteomics.

ABSTRACT: Protein adsorption to the surface of a nanoparticle can fundamentally alter the character, behavior, and fate of a nanoparticle in vivo. Current methods to capture the protein corona rely on physical separation techniques and are unable to resolve key, individual protein-nanoparticle interactions. As a result, the precise link between the "synthetic" and the "biological" identity of a nanoparticle remains unclear. Herein, we report an unbiased photoaffinity-based approach to capture, characterize, and quantify the protein corona of liposomes in their native state. Compared to conventional methods, our photoaffinity approach reveals markedly different interacting proteins as well as reduced total protein binding to liposome surfaces. Identified proteins do not follow protein abundancy patte

SUBMITTER: Pattipeiluhu R 

PROVIDER: S-EPMC7181318 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature