Zebrafish etv2 knock-in line labels vascular endothelial and blood progenitor cells.

ABSTRACT:

Background

ETS transcription factor Etv2/Etsrp is one of the earliest markers for vascular and hematopoietic progenitors and functions as a key regulator of hematovascular development in multiple vertebrates, including zebrafish. Therefore, transgenic etv2 reporter lines provide a valuable tool to study vasculogenesis and hematopoiesis. However, previously generated zebrafish reporter lines do not fully recapitulate the endogenous pattern of etv2 expression.

Results

Here we used CRISPR/Cas9-mediated homology-independent DNA repair approach to knock-in a Gal4 transcriptional activator into the zebrafish etv2 genomic locus, thus generating etv2 ^ci32Gt gene trap line. etv2 ^ci32Gt ; UAS:GFP embryos show GFP expression in vascular endothelial, myeloid and re

SUBMITTER: Chestnut B 

PROVIDER: S-EPMC7181369 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature