Vitamin lipid nanoparticles enable adoptive macrophage transfer for the treatment of multidrug-resistant bacterial sepsis.

ABSTRACT: Sepsis, a condition caused by severe infections, affects more than 30 million people worldwide every year and remains the leading cause of death in hospitals^1,2. Moreover, antimicrobial resistance has become an additional challenge in the treatment of sepsis³, and thus, alternative therapeutic approaches are urgently needed^2,3. Here, we show that adoptive transfer of macrophages containing antimicrobial peptides linked to cathepsin B in the lysosomes (MACs) can be applied for the treatment of multidrug-resistant bacteria-induced sepsis in mice with immunosuppression. The MACs are constructed by transfection of vitamin C lipid nanoparticles that deliver antimicrobial peptide and cathepsin B (AMP-CatB) mRNA. The vitamin C lipid nanoparticles allow the specifi

SUBMITTER: Hou X 

PROVIDER: S-EPMC7181370 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature