Improvement of sabinene tolerance of Escherichia coli using adaptive laboratory evolution and omics technologies.

ABSTRACT:

Background

Biosynthesis of sabinene, a bicyclic monoterpene, has been accomplished in engineered microorganisms by introducing heterologous pathways and using renewable sugar as a carbon source. However, the efficiency and titers of this method are limited by the low host tolerance to sabinene (in both eukaryotes and prokaryotes).

Results

In this study, Escherichia coli BL21(DE3) was selected as the strain for adaptive laboratory evolution. The strain was evolved by serial passaging in the medium supplemented with gradually increasing concentration of sabinene, and the evolved strain XYF(DE3), which exhibited significant tolerance to sabinene, was obtained. Then, XYF(DE3) was used as the host for sabinene production and an 8.43-fold higher sabinene production was achi

SUBMITTER: Wu T 

PROVIDER: S-EPMC7181518 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature