Cell free DNA from respiratory pathogens is detectable in the blood plasma of Cystic Fibrosis patients.

ABSTRACT: Diagnostically informative microbial cell-free DNA (cfDNA) can be detected from blood plasma during fulminant infections such as sepsis. However, the potential for DNA from airway pathogens to enter the circulation of cystic fibrosis (CF) patients during chronic infective states has not yet been evaluated. We assessed whether patient blood contained measurable quantities of cfDNA from CF respiratory microorganisms by sequencing plasma from 21 individuals with CF recruited from outpatient clinics and 12 healthy controls. To account for possible contamination with exogenous microbial nucleic acids, statistical significance of microbe-derived read counts from CF patients was determined relative to the healthy control population. In aggregate, relative abundance of microbial cfDNA was nearly a

SUBMITTER: Barrett SLR 

PROVIDER: S-EPMC7181635 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature