Nitrogen starvation reveals the mitotic potential of mutants in the S/MAPK pathways.

ABSTRACT: The genetics of quiescence is an emerging field compared to that of growth, yet both states generate spontaneous mutations and genetic diversity fueling evolution. Reconciling mutation rates in dividing conditions and mutation accumulation as a function of time in non-dividing situations remains a challenge. Nitrogen-starved fission yeast cells reversibly arrest proliferation, are metabolically active and highly resistant to a variety of stresses. Here, we show that mutations in stress- and mitogen-activated protein kinase (S/MAPK) signaling pathways are enriched in aging cultures. Targeted resequencing and competition experiments indicate that these mutants arise in the first month of quiescence and expand clonally during the second month at the expense of the parental population. Reconst

SUBMITTER: Makarenko R 

PROVIDER: S-EPMC7181643 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature