A Yeast System for Discovering Optogenetic Inhibitors of Eukaryotic Translation Initiation.

ABSTRACT: The precise spatiotemporal regulation of protein synthesis is essential for many complex biological processes such as memory formation, embryonic development, and tumor formation. Current methods used to study protein synthesis offer only a limited degree of spatiotemporal control. Optogenetic methods, in contrast, offer the prospect of controlling protein synthesis noninvasively within minutes and with a spatial scale as small as a single synapse. Here, we present a hybrid yeast system where growth depends on the activity of human eukaryotic initiation factor 4E (eIF4E) that is suitable for screening optogenetic designs for the down-regulation of protein synthesis. We used this system to screen a diverse initial panel of 15 constructs designed to couple a light switchable domain (PYP, RsL

SUBMITTER: Lu H 

PROVIDER: S-EPMC7181834 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature