Overexpression of PtHMGR enhances drought and salt tolerance of poplar.

ABSTRACT:

Background and aims

Soil salinization and aridification are swiftly engulfing the limited land resources on which humans depend, restricting agricultural production. Hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) is important in the biosynthesis of terpenoids, which are involved in plant growth, development and responses to environmental stresses. This study aimed to provide guidance for producing salt- and drought-resistant poplar.

Methods

A protein expression system was used to obtain PtHMGR protein, and high-performance liquid chromatography was used to detect the activity of PtHMGR protein in vitro. In addition, a simplified version of the leaf infection method was used for transformation of 'Nanlin895' poplar (Populus×euramericana). qRT-PCR was used to identify ex

SUBMITTER: Wei H 

PROVIDER: S-EPMC7182595 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature