Fluorescent substrates for haloalkane dehalogenases: Novel probes for mechanistic studies and protein labeling.

ABSTRACT: Haloalkane dehalogenases are enzymes that catalyze the cleavage of carbon-halogen bonds in halogenated compounds. They serve as model enzymes for studying structure-function relationships of >100.000 members of the α/β-hydrolase superfamily. Detailed kinetic analysis of their reaction is crucial for understanding the reaction mechanism and developing novel concepts in protein engineering. Fluorescent substrates, which change their fluorescence properties during a catalytic cycle, may serve as attractive molecular probes for studying the mechanism of enzyme catalysis. In this work, we present the development of the first fluorescent substrates for this enzyme family based on coumarin and BODIPY chromophores. Steady-state and pre-steady-state kinetics with two of the most active haloalkane d

SUBMITTER: Dockalova V 

PROVIDER: S-EPMC7182704 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature