Obesity significantly alters the human sperm proteome, with potential implications for fertility.

ABSTRACT:

Purpose

In men, obesity may lead to poor semen parameters and reduced fertility. However, the causative links between obesity and male infertility are not totally clear, particularly on a molecular level. As such, we investigated how obesity modifies the human sperm proteome, to elucidate any important implications for fertility.

Methods

Sperm protein lysates from 5 men per treatment, classified as a healthy weight (body mass index (BMI) ≤ 25 kg/m²) or obese (BMI ≥ 30 kg/m²), were FASP digested, submitted to liquid chromatography tandem mass spectrometry, and compared by label-free quantification. Findings were confirmed for several proteins by qualitative immunofluorescence and a quantitative protein immunoassay.

Results

A total of 2034 proteins

SUBMITTER: Pini T 

PROVIDER: S-EPMC7183029 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature