The growth and development conditions in mouse offspring derived from ovarian tissue cryopreservation and orthotopic transplantation.

ABSTRACT:

Purpose

To investigate the potential development or metabolic risk in offspring derived from mice with transplanted frozen-thawed ovarian tissue.

Methods

Mice ovaries were intervened by vitrification (group V) and slow-freezing (group S) cryopreservation and orthotopic transplantation. Orthotopic transplantation of fresh ovarian (group F) and natural mating (group C) served as control groups. The fertility restoration and health conditions of generations were assessed by offspring counts, anti-fatigue and motor ability, and organ morphology. The methylation rate and expression level of imprinted genes (IGF2R, H19, SNRPN, and PLAGL1) were used to predict the potential risk of development in transplanted generations.

Results

Both the percentage of normal morphological f

SUBMITTER: Yan Z 

PROVIDER: S-EPMC7183038 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature