The corepressor NCOR1 and OCT4 facilitate early reprogramming by suppressing fibroblast gene expression.

ABSTRACT: Reprogramming somatic cells to induced pluripotent stem cells (iPSC) succeeds only in a small fraction of cells within the population. Reprogramming occurs in distinctive stages, each facing its own bottlenecks. It initiates with overexpression of transcription factors OCT4, SOX2, KLF4 and c-MYC (OSKM) in somatic cells such as mouse embryonic fibroblasts (MEFs). OSKM bind chromatin, silencing the somatic identity and starting the stepwise reactivation of the pluripotency programme. However, inefficient suppression of the somatic lineage leads to unwanted epigenetic memory from the tissue of origin, even in successfully generated iPSCs. Thus, it is essential to shed more light on chromatin regulators and processes involved in dissolving the somatic identity. Recent work characterised the ro

SUBMITTER: Penalosa-Ruiz G 

PROVIDER: S-EPMC7183309 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature