Comparative transcriptomic analysis of vascular endothelial cells after hypoxia/re-oxygenation induction based on microarray technology.

ABSTRACT:

Objective

To provide comprehensive data to understand mechanisms of vascular endothelial cell (VEC) response to hypoxia/re-oxygenation.

Methods

Human umbilical vein endothelial cells (HUVECs) were employed to construct hypoxia/re-oxygenation-induced VEC transcriptome profiling. Cells incubated under 5% O₂, 5% CO₂, and 90% N₂ for 3 h followed by 95% air and 5% CO₂ for 1 h were used in the hypoxia/re-oxygenation group. Those incubated only under 95% air and 5% CO₂ were used in the normoxia control group.

Results

By using a well-established microarray chip consisting of 58 339 probes, the study identified 372 differentially expressed genes. While part of the genes are known to be VEC hypoxia/re-oxygenation-related, s

SUBMITTER: Xu J 

PROVIDER: S-EPMC7183444 | biostudies-literature | 2020 Apr.

REPOSITORIES: biostudies-literature