Differential annotation of converted metabolites (DAC-Met): Exploration of Maoto (Ma-huang-tang)-derived metabolites in plasma using high-resolution mass spectrometry.

ABSTRACT:

Introduction

Traditional herbal medicine (THM) contains a vast number of natural compounds with varying degrees of pharmacological activity. To elucidate the mode of action, comprehensive metabolite profiling in the plasma before and after administration of THM is essential.

Objective

The aim of this study was to explore and identify/annotate converted metabolites after administration of THM in humans.

Methods

We performed untargeted metabolome analysis of human plasma collected before and after administration of maoto (ma-huang-tang), a traditional Japanese Kampo medicine. Maoto-derived metabolites were then selected and annotated following the DAC-Met strategy, which is an annotation method that uses mass differences of major metabolic reactions among the detected p

SUBMITTER: Ohbuchi K 

PROVIDER: S-EPMC7183508 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature