Clonorchis sinensis secretory protein CsAg17 vaccine induces immune protection.

ABSTRACT:

Background

Clonorchiasis is endemic in East and Southeast Asian countries. For a preventive strategy against infectious diseases, vaccination is the most effective. Here, we evaluated the molecular characteristics and immune responses of CsAg17 protein from Clonorchis sinensis, and investigated its protective effects against C. sinensis challenge.

Methods

A cDNA clone encoding CsAg17 protein and containing a secretory signal peptide at the N-terminus was retrieved from the C. sinensis transcriptome bank. Recombinant CsAg17 B-cell epitope protein and cDNA vaccines were produced and their immune responses were evaluated in FVB mice. The proportional changes of CD3⁺/CD4⁺ and CD3⁺/CD8⁺ T cells were detected by flow cytometry, and immun

SUBMITTER: Bai X 

PROVIDER: S-EPMC7183723 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature