Cutting off ciliary protein import: intraflagellar transport after dendritic femtosecond-laser ablation.

ABSTRACT: Primary cilia, organelles protruding from the surface of eukaryotic cells, act as cellular antennae to detect and transmit signals from the extracellular environment. They are built and maintained by continuous cycles of intraflagellar transport (IFT), where ciliary proteins are transported between the ciliary base and tip. These proteins originate from the cell body because cilia lack protein synthesis machinery. How input from the cell body affects IFT and ciliary function is not well understood. Here, we use femtosecond-laser ablation to perturb the dendritic input of proteins to chemosensory cilia in living Caenorhabditis elegans. Using fluorescence microscopy, we visualize and quantify the real-time response of ciliary proteins to dendritic ablation. We find that the res

SUBMITTER: Mijalkovic J 

PROVIDER: S-EPMC7183794 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature