Cholesterol Biosynthesis and Uptake in Developing Neurons.

ABSTRACT: Brain cholesterol biosynthesis, a separate and distinct process from whole-body cholesterol homeostasis, starts during embryonic development. To gain a better understanding of the neuronal and glial contributions to the brain cholesterol pool, we studied this process in control, Dhcr7^-/-, and Dhcr24^-/- cell cultures. Our LC-MS/MS method allowed us to measure several different sterol intermediates and cholesterol during neuronal differentiation. We found that developing cortical neurons rely on endogenous cholesterol synthesis and utilize ApoE-complexed cholesterol and sterol precursors from their surroundings. Both developing neurons and astrocytes release cholesterol into their local environment. Our studies also uncovered that developing

SUBMITTER: Genaro-Mattos TC 

PROVIDER: S-EPMC7184320 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature