Immunoglobulin expression and the humoral immune response is regulated by the non-canonical poly(A) polymerase TENT5C.

ABSTRACT: TENT5C is a non-canonical cytoplasmic poly(A) polymerase highly expressed by activated B cells to suppress their proliferation. Here we measure the global distribution of poly(A) tail lengths in responsive B cells using a Nanopore direct RNA-sequencing approach, showing that TENT5C polyadenylates immunoglobulin mRNAs regulating their half-life and consequently steady-state levels. TENT5C is upregulated in differentiating plasma cells by innate signaling. Compared with wild-type, Tent5c^-/- mice produce fewer antibodies and have diminished T-cell-independent immune response despite having more CD138^high plasma cells as a consequence of accelerated differentiation. B cells from Tent5c^-/- mice also have impaired capacity of the secretory pathway, with reduced E

SUBMITTER: Bilska A 

PROVIDER: S-EPMC7184606 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature