Synchronous, Crosstalk-free Correlative AFM and Confocal Microscopies/Spectroscopies.

ABSTRACT: Microscopies have become pillars of our characterization tools to observe biological systems and assemblies. Correlative and synchronous use of different microscopies relies on the fundamental assumption of non-interference during images acquisitions. In this work, by exploring the correlative use of Atomic Force Microscopy and confocal-Fluorescence-Lifetime Imaging Microscopy (AFM-FLIM), we quantify cross-talk effects occurring during synchronous acquisition. We characterize and minimize optomechanical forces on different AFM cantilevers interfering with normal AFM operation as well as spurious luminescence from the tip and cantilever affecting time-resolved fluorescence detection. By defining non-interfering experimental imaging parameters, we show accurate real-time acquisition and two-

SUBMITTER: Fernandes TFD 

PROVIDER: S-EPMC7184616 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature