First steps towards combining faecal immunochemical testing with the gut microbiome in colorectal cancer screening.

ABSTRACT:

Objectives

Many countries use faecal immunochemical testing (FIT) to screen for colorectal cancer. There is increasing evidence that faecal microbiota play a crucial role in colorectal cancer carcinogenesis. We assessed the possibility of measuring faecal microbial features in FIT as potential future biomarkers in colorectal cancer screening.

Methods

Bacterial stability over time and the possibility of bacterial contamination were evaluated using quantitative polymerase chain reaction analysis. Positive FIT samples (n = 200) of an average-risk screening cohort were subsequently analysed for universal 16S, and bacteria. Escherichia coli (E. coli), Fusobacterium nucleatum (F. nucleatum), Bacteroidetes and Faecalibacterium prausnitzi

SUBMITTER: Grobbee EJ 

PROVIDER: S-EPMC7184657 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature