Ex vivo culture of mouse skin activates an interleukin 1 alpha-dependent inflammatory response.

ABSTRACT: Ex vivo culture of mouse and human skin causes an inflammatory response characterized by production of multiple cytokines. We used ex vivo culture of mouse tail skin specimens to investigate mechanisms of this skin culture-induced inflammatory response. Multiplex assays revealed production of interleukin 1 alpha (IL-1α), interleukin 1 beta (IL-1β), interleukin 6 (IL-6), chemokine C-X-C motif ligand 1 (CXCL1), granulocyte colony-stimulating factor (G-CSF) and granulocyte-macrophage colony-stimulating factor (GM-CSF) during skin culture, and quantitative PCR revealed transcripts for these proteins were also increased. Ex vivo cultures of skin from myeloid differentiation primary response 88 deficient mice (Myd88^-/- ) demonstrated significantly reduced expression of transcripts for

SUBMITTER: Zhou HM 

PROVIDER: S-EPMC7184933 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature