TET1 regulates DNA repair in human glial cells.

ABSTRACT: Glioblastomas are the most aggressive of malignant brain cancers with a median patient survival of approximately 18 months. We recently demonstrated that Tet methylcytosine dioxygenase 1(TET1) is involved in cellular responses to ionizing radiation (IR) in glial-, glioblastoma-, and non-tumor-derived cells. This study used a lentiviral-mediated knockdown of TET1 to further dissect the contribution of TET1 to the DNA damage response in glial cell lines by evaluating its role in DNA repair. TET1-deficient glial cell lines displayed attenuated cytotoxicity compared to non-targeted knockdown after treatment with IR but these differences were not observed between control and TET1 deficient in response to inhibitors of Na⁺/K⁺-ATPase. Additionally, the percentage of glial ce

SUBMITTER: Kuhns KJ 

PROVIDER: S-EPMC7184935 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature