The human papillomavirus confers radiosensitivity in oropharyngeal cancer cells by enhancing DNA double strand break.

ABSTRACT: Background: Patients with Human papillomavirus (HPV)-positive oropharyngeal squamous cell carcinoma (OPSCC) has better outcomes than those with HPV-negative OPSCC. This may be related to its enhanced radiosensitivity. This study examined the effect of HPV and its E6 oncoprotein on the morphology, radiosensitivity, and repair of radiation-induced DNA damage. Materials and Methods: HPV-negative UM-SCC4 with and without transfection of HPV E6 oncoprotein, HPV-negative UPCI-SCC-089 and HPV-positive UPCI-SCC-099 cell lines were used in this study. The radiosensitivity and morphological changes after radiation were determined by clonogenic assay. Radiation-induced double-strand breaks in the DNA was measured by γ-H2AX foci immunofluorescent assay. Results: The survival fraction after 10 Gy was s

SUBMITTER: Zhang M 

PROVIDER: S-EPMC7185066 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature