A complex regulatory landscape involved in the development of mammalian external genitals.

ABSTRACT: Developmental genes are often controlled by large regulatory landscapes matching topologically associating domains (TADs). In various contexts, the associated chromatin backbone is modified by specific enhancer-enhancer and enhancer-promoter interactions. We used a TAD flanking the mouse HoxD cluster to study how these regulatory architectures are formed and deconstructed once their function achieved. We describe this TAD as a functional unit, with several regulatory sequences acting together to elicit a transcriptional response. With one exception, deletion of these sequences didn't modify the transcriptional outcome, a result at odds with a conventional view of enhancer function. The deletion and inversion of a CTCF site located near these regulatory sequences did not affect trans

SUBMITTER: Amandio AR 

PROVIDER: S-EPMC7185996 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature