Marked Up-Regulation of ACE2 in Hearts of Patients With Obstructive Hypertrophic Cardiomyopathy: Implications for SARS-CoV-2-Mediated COVID-19.

ABSTRACT:

Objective

To explore the transcriptomic differences between patients with hypertrophic cardiomyopathy (HCM) and controls.

Patients and methods

RNA was extracted from cardiac tissue flash frozen at therapeutic surgical septal myectomy for 106 patients with HCM and 39 healthy donor hearts. Expression profiling of 37,846 genes was performed using the Illumina Human HT-12v3 Expression BeadChip. All patients with HCM were genotyped for pathogenic variants causing HCM. Technical validation was performed using quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot. This study was started on January 1, 1999, and final analysis was completed on April 20, 2020.

Results

Overall, 22% of the transcriptome (8443 of 37,846 genes) was expressed differentially bet

SUBMITTER: Bos JM 

PROVIDER: S-EPMC7186205 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature