Alpha1-antitrypsin ameliorates islet amyloid-induced glucose intolerance and β-cell dysfunction.

ABSTRACT:

Objective

Pancreatic β-cell failure is central to the development and progression of type 2 diabetes (T2D). The aggregation of human islet amyloid polypeptide (hIAPP) has been associated with pancreatic islet inflammation and dysfunction in T2D. Alpha1-antitrypsin (AAT) is a circulating protease inhibitor with anti-inflammatory properties. Here, we sought to investigate the potential therapeutic effect of AAT treatment in a mouse model characterized by hIAPP overexpression in pancreatic β-cells.

Methods

Mice overexpressing hIAPP (hIAPP-Tg) in pancreatic β-cells were used as a model of amyloid-induced β-cell dysfunction. Glucose homeostasis was evaluated by glucose tolerance tests and insulin secretion assays. Apoptosis and amyloid formation was assessed in hIAPP-Tg mouse isl

SUBMITTER: Rodriguez-Comas J 

PROVIDER: S-EPMC7186564 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature