Developing a newborn rat model of ventriculitis without concomitant bacteremia by intraventricular injection of K1 (-) Escherichia coli.

ABSTRACT:

Background

Neonatal meningitis caused by Escherichia coli results in high mortality and neurological disabilities, and the concomitant systemic bacteremia confounds its mortality and brain injury. This study developed an experimental model of neonatal ventriculitis without concomitant systemic bacteremia by determining the bacterial inoculum of K1 capsule-negative E. coli by intraventricular injection in newborn rats.

Methods

We carried out intraventricular injections 1 × 10² (low dose), 5 × 10² (medium dose), or 1 × 10³ (high dose) colony-forming units (CFU) of K1 (-) E. coli (EC5ME) in Sprague-Dawley rats at postnatal day (P) 11. Ampicillin was started at P12. Blood and cerebrospinal fluid (CSF) cultures were performed at 6 h, 1 day, and 6 

SUBMITTER: Ahn SY 

PROVIDER: S-EPMC7187168 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature