Stable Prenucleation Calcium Carbonate Clusters Define Liquid-Liquid Phase Separation.

ABSTRACT: Liquid-liquid phase separation (LLPS) is an intermediate step during the precipitation of calcium carbonate, and is assumed to play a key role in biomineralization processes. Here, we have developed a model where ion association thermodynamics in homogeneous phases determine the liquid-liquid miscibility gap of the aqueous calcium carbonate system, verified experimentally using potentiometric titrations, and kinetic studies based on stopped-flow ATR-FTIR spectroscopy. The proposed mechanism explains the variable solubilities of solid amorphous calcium carbonates, reconciling previously inconsistent literature values. Accounting for liquid-liquid amorphous polymorphism, the model also provides clues to the mechanism of polymorph selection. It is general and should be tested for systems othe

SUBMITTER: Avaro JT 

PROVIDER: S-EPMC7187218 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature