CDC2 and plasmacytoid dendritic cells diminish from tissues of patients with non-Hodgkin orbital lymphoma and idiopathic orbital inflammation.

ABSTRACT: Non-Hodgkin orbital lymphoma (NHOL) and idiopathic orbital inflammation (IOI) are common orbital conditions with largely unknown pathophysiology. To investigate the immune cell composition of these diseases, we performed standardized 29 parameter flow cytometry phenotyping in peripheral blood mononuclear cells of 18 NHOL patients, 21 IOI patients, and 41 unaffected controls. Automatic gating by FlowSOM revealed decreased abundance of meta-clusters containing dendritic cells in patients, which we confirmed by manual gating. A decreased percentage of (HLA-DR⁺ CD303⁺ CD123⁺ ) plasmacytoid dendritic cells (pDC) in the circulation of IOI patients and decreased (HLA-DR⁺ CD11c⁺ CD1c⁺ ) conventional dendritic cells (cDC) type-2 for

SUBMITTER: Laban KG 

PROVIDER: S-EPMC7187234 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature