Proteomic and transcriptomic profiling of Pten gene-knockout mouse model of prostate cancer.

ABSTRACT:

Background

The prostate-specific phosphatase and tensin homolog deleted on chromosome 10 (Pten) gene-conditional knockout (KO) mouse carcinogenesis model is highly desirable for studies of prostate cancer biology and chemoprevention due to its close resemblance of primary molecular defect and many histopathological features of human prostate cancer including androgen response and disease progression from prostatic intraepithelial neoplasia to invasive adenocarcinoma. Here, we profiled the proteome and transcriptome of the Pten-KO mouse prostate tumors for global macromolecular expression alterations for signaling changes and biomarker signatures.

Methods

For proteomics, four pairs of whole prostates from tissue-specific conditional knockout Pten-KO mice (12-15 weeks of age)

SUBMITTER: Zhang J 

PROVIDER: S-EPMC7187266 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature