Rapid development of clone-specific, high-performing perfusion media from established feed supplements.

ABSTRACT: Perfusion cultivation of recombinant CHO cells is of substantial interest to the biopharmaceutical industry. This is due to increased space-time-yields (STYs) and a short residence time of the recombinant protein in the bioreactor. Economic processes rely on cultivation media supporting rapid growth in the exponential phase and high protein production in the stationary phase at minimal media consumption rates. To develop clone-specific, high-performing perfusion media we present a straightforward and rapid two-step approach combining commercially available basal media and feed supplements using design-of-experiment. First, the best performing feed supplements are selected in batch cultures. Then, the mixing ratio of selected feed supplements is optimized in small-scale semicontinuous perfu

SUBMITTER: Mayrhofer P 

PROVIDER: S-EPMC7187557 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature