Circulating cell free DNA during definitive chemo-radiotherapy in non-small cell lung cancer patients - initial observations.

ABSTRACT:

Background

The overall aim was to investigate the change over time in circulating cell free DNA (cfDNA) in patients with locally advanced non-small cell lung cancer (NSCLC) undergoing concurrent chemo-radiotherapy. Furthermore, to assess the possibility of detecting circulating cell free tumor DNA (ctDNA) using shallow whole genome sequencing (sWGS) and size selection.

Methods

Ten patients were included in a two-phase study. The first four patients had blood samples taken prior to a radiation therapy (RT) dose fraction and at 30 minutes, 1 hour and 2 hours after RT to estimate the short-term dynamics of cfDNA concentration after irradiation. The remaining six patients had one blood sample taken on six treatment days 30 minutes post treatment to measure cfDNA levels. Presence

SUBMITTER: Nygard L 

PROVIDER: S-EPMC7188247 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature