The TIR-domain containing effectors BtpA and BtpB from Brucella abortus impact NAD metabolism.

ABSTRACT: Brucella species are facultative intracellular Gram-negative bacteria relevant to animal and human health. Their ability to establish an intracellular niche and subvert host cell pathways to their advantage depends on the delivery of bacterial effector proteins through a type IV secretion system. Brucella Toll/Interleukin-1 Receptor (TIR)-domain-containing proteins BtpA (also known as TcpB) and BtpB are among such effectors. Although divergent in primary sequence, they interfere with Toll-like receptor (TLR) signaling to inhibit the innate immune responses. However, the molecular mechanisms implicated still remain unclear. To gain insight into the functions of BtpA and BtpB, we expressed them in the budding yeast Saccharomyces cerevisiae as a eukaryotic cell model. We found that both effec

SUBMITTER: Coronas-Serna JM 

PROVIDER: S-EPMC7188309 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature