An Ultrasensitive Fluorescence Assay for the Detection of Halides and Enzymatic Dehalogenation.

ABSTRACT: Halide assays are important for the study of enzymatic dehalogenation, a topic of great industrial and scientific importance. Here we describe the development of a very sensitive halide assay that can detect less than a picomole of bromide ions, making it very useful for quantifying enzymatic dehalogenation products. Halides are oxidised under mild conditions using the vanadium-dependent chloroperoxidase from Curvularia inaequalis, forming hypohalous acids that are detected using aminophenyl fluorescein. The assay is up to three orders of magnitude more sensitive than currently available alternatives, with detection limits of 20 nM for bromide and 1 μM for chloride and iodide. We demonstrate that the assay can be used to determine specific activities of dehalogenases and validate this by c

SUBMITTER: Aslan-Uzel AS 

PROVIDER: S-EPMC7188320 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature