The dynamics of DNA methylation during epigenetic reprogramming of primordial germ cells in medaka (<i>Oryzias latipes</i>).

ABSTRACT: Primordial germ cells (PGCs) are precursors of eggs and sperm. How the PGCs epigenetically reprogram during early embryonic development in fish is currently unknown. Here we generated a series of PGC methylomes using whole genome bisulfite sequencing across key stages from 8 days post fertilization (dpf) to 25 dpf coinciding with germ cell sex determination and gonadal sex differentiation in medaka (Oryzias latipes) to elucidate the dynamics of DNA methylation during epigenetic reprogramming in germ cells. Our high-resolution DNA methylome maps show a global demethylation taking place in medaka PGCs in a two-step strategy. The first step occurs between the blastula and 8-dpf stages, and the second step occurs between the 10-dpf and 12-dpf stages. Both demethylation processes are glo

SUBMITTER: Wang X 

PROVIDER: S-EPMC7188396 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature