Rapid identification of highly potent human anti-GPCR antagonist monoclonal antibodies.

ABSTRACT: Complex cellular targets such as G protein-coupled receptors (GPCRs), ion channels, and other multi-transmembrane proteins represent a significant challenge for therapeutic antibody discovery, primarily because of poor stability of the target protein upon extraction from cell membranes. To assess whether a limited set of membrane-bound antigen formats could be exploited to identify functional antibodies directed against such targets, we selected a GPCR of therapeutic relevance (CCR1) and identified target binders using an in vitro yeast-based antibody discovery platform (Adimab^TM) to expedite hit identification. Initially, we compared two different biotinylated antigen formats overexpressing human CCR1 in a 'scouting' approach using a subset of the antibody library. Binde

SUBMITTER: Scott MJ 

PROVIDER: S-EPMC7188403 | biostudies-literature | 2020 Jan-Dec

REPOSITORIES: biostudies-literature