A novel model of liver cancer stem cells developed from induced pluripotent stem cells.

ABSTRACT:

Background

Liver cancer is the second most common cause of cancer-related death. Every type of tumours including liver cancer contains cancer stem cells (CSCs). To date, the molecular mechanism regulating the development of liver CSCs remains unknown.

Methods

In this study, we tried to generate a new model of liver CSCs by converting mouse induced pluripotent stem cells (miPSCs) with hepatocellular carcinoma (HCC) cell line Huh7 cells conditioned medium (CM). miPSCs treated with CM were injected into the liver of BALB/c nude mice. The developed tumours were then excised and analysed.

Results

The primary cultured cells from the malignant tumour possessed self-renewal capacity, differentiation potential and tumorigenicity in vivo, which were found rich in liver cancer-a

SUBMITTER: Afify SM 

PROVIDER: S-EPMC7188674 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature