Artifact-free and high-temporal-resolution in vivo opto-electrophysiology with microLED optoelectrodes.

ABSTRACT: The combination of in vivo extracellular recording and genetic-engineering-assisted optical stimulation is a powerful tool for the study of neuronal circuits. Precise analysis of complex neural circuits requires high-density integration of multiple cellular-size light sources and recording electrodes. However, high-density integration inevitably introduces stimulation artifact. We present minimal-stimulation-artifact (miniSTAR) μLED optoelectrodes that enable effective elimination of stimulation artifact. A multi-metal-layer structure with a shielding layer effectively suppresses capacitive coupling of stimulation signals. A heavily boron-doped silicon substrate silences the photovoltaic effect induced from LED illumination. With transient stimulation pulse shaping, we reduced stimulation

SUBMITTER: Kim K 

PROVIDER: S-EPMC7188816 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature