The CDK inhibitor p57^Kip2 enhances the activity of the transcriptional coactivator FHL2.

ABSTRACT: The eukaryotic cell cycle is negatively regulated by cyclin-dependent kinase inhibitors (CKIs). p57^Kip2 is a member of the Cip/Kip family of CKIs and frequently inactivated by genomic mutations associated with human overgrowth disorders. There is increasing evidence for p57 to control cellular processes in addition to cell cycle and CDK regulation including transcription, apoptosis, migration or development. In order to obtain molecular insights to unknown functions of p57, we performed a protein interaction screen. We identified the transcription regulator four-and-a-half LIM-only protein 2 (FHL2) as a novel p57-binding protein. Co-immunoprecipitation and reporter gene assays were used to elucidate the physiological and functional relevance of p57/FHL2 interaction. We found in

SUBMITTER: Kullmann MK 

PROVIDER: S-EPMC7188849 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature