The downregulated membrane expression of CD18 in CD34⁺ cells defines a primitive population of human hematopoietic stem cells.

ABSTRACT:

Background

CD18 is the common beta subunit of β₂ integrins, which are expressed on hematopoietic cells. β₂ integrins are essential for cell adhesion and leukocyte trafficking.

Methods

Here we have analyzed the expression of CD18 in different subsets of human hematopoietic stem and progenitor cells (HSPCs) from cord blood (CB), bone marrow (BM), and mobilized peripheral blood (mPB) samples. CD34⁺ cells were classified into CD18^high and CD18^low/neg, and each of these populations was analyzed for the expression of HSPC markers, as well as for their clonogenity, quiescence state, and repopulating ability in immunodeficient mice.

Results

A downregulated membrane expression of CD18 was associated with a primitive hemato

SUBMITTER: Mesa-Nunez C 

PROVIDER: S-EPMC7189462 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature