LSD1 inhibition yields functional insulin-producing cells from human embryonic stem cells.

ABSTRACT:

Background

Human embryonic stem cells represent a potentially unlimited source of insulin-producing cells for diabetes therapy. While tremendous progress has been made in directed differentiation of human embryonic stem cells into IPCs in vitro, the mechanisms controlling its differentiation and function are not fully understood. Previous studies revealed that lysine-specific demethylase 1(LSD1) balanced the self-renewal and differentiation in human induced pluripotent stem cells and human embryonic stem cells. This study aims to explore the role of LSD1 in directed differentiation of human embryonic stem cells into insulin-producing cells.

Methods

Human embryonic stem cell line H9 was induced into insulin-producing cells by a four-step differentiation protocol. Lentivirus t

SUBMITTER: He F 

PROVIDER: S-EPMC7189473 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature