A Rapid and High Throughput MIC Determination Method to Screen Uranium Resistant Microorganisms.

ABSTRACT: The assessment of minimum inhibitory concentration (MIC) is a conventional technique used for the screening of microbial resistance against antibiotics, biocides, and contaminants such as heavy metals. However, as part of our ongoing work, we have observed biases associated with using traditional liquid MIC method to screen microbial heavy metal resistance, including both bacterial and fungal strains. Specifically, the addition of uranium into synthetic media causes immediate precipitation prior to the initiation of microbial growth, thus hampering the optical density measurements, and the obtained MIC values are thus flawed and inaccurate. To address this discrepancy, we report the optimization and development of a serial-dilution-based MIC method conducted on solid growth media supplemen

SUBMITTER: Agarwal M 

PROVIDER: S-EPMC7189662 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature