Exploratory analysis of age and sex dependent DNA methylation patterns on the X-chromosome in whole blood samples.

ABSTRACT:

Background

Large numbers of autosomal sites are found differentially methylated in the aging genome. Due to analytical difficulties in dealing with sex differences in X-chromosome content and X-inactivation (XCI) in females, this has not been explored for the X chromosome.

Methods

Using data from middle age to elderly individuals (age 55+ years) from two Danish cohorts of monozygotic twins and the Scottish Lothian Birth Cohort 1921, we conducted an X-chromosome-wide analysis of age-associated DNA methylation patterns with consideration of stably inferred XCI status.

Results

Through analysing and comparing sex-specific X-linked DNA methylation changes over age late in life, we identified 123, 293 and 55 CpG sites significant (FDR < 0.05) only in males, only in females

SUBMITTER: Li S 

PROVIDER: S-EPMC7189689 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature