Solubilization of Human Interferon β-1b Inclusion Body Proteins by Organic Solvents.

ABSTRACT: Purposes: Solubilization of inclusion bodies expressed in E. coli is a critical step during manufacturing of recombinant proteins expressed as inclusion bodies. So far, various methods have been used for solubilization and purification of inclusion body proteins to obtain active proteins with high purity and yield. The aim of this study was to examine the benefit of organic solvents such as alcohols in solubilization of recombinant interferon β-1b inclusion bodies. Methods: Effect of important parameters inclusion pH, concentration and type of denaturant and concentration of alcoholic solvents were optimized to formulate a suitable solubilization buffer and investigate their effect on solubilization of interferon β-1b inclusion bodies. Results: Our findings showed the acidic pH in the rang

SUBMITTER: Nekoufar S 

PROVIDER: S-EPMC7191233 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature